Hardware and software integration and testing for the automation of bright-field microscopy for tuberculosis detection

Automated microscopy for the detection of tuberculosis (TB) in sputum smears would reduce the load on technicians, especially in countries with a high TB burden. This dissertation reports on the development and testing of an automated system built around a conventional microscope for the detection of TB in Ziehl-Neelsen (ZN) stained sputum smears. Microscope auto-focusing, image analysis and stage movement were integrated. Images were captured at 40x magnification

A study on types of hydrogen bonding in crystal structure of 1-acetyl-3-thiosemicarbazide

592-596The unit cell structure of 1-acetyl-3-thiosemicarbazide (ATSC) and the types of hydrogen bonds present in crystalline form are envisaged from single crystal X-ray diffraction studies. The presence of four types of hydrogen bonds in crystal packing is evident (N─H• • •O, N─H• • •N, N─H• • •S and O─H• • •O) from the results obtained in present study. The topological analysis of hydrogen bonds indicated both inter- and intramolecular hydrogen bonding. Presence of water molecule is evident in its structure indicating that the title compound is in hydrated form. The single crystal X-ray diffraction data corresponding to hydrogen bonding reveals the hydrogen-bond strength sequence as: N─H• • •N > O─H• • •O > N─H• • •O > N─H• • •S